Isolation of Clostridium difficile from chicken meat sold in meat stores of Isfahan City

Clostridium difficile is a hot-positive, anaerobic spore regarded as important pathogens in human pathogenesis. Clostridium difficile is a nosocomial pathogen associated with diarrhea and colitis (inflammation of the colon) in patients admitted to hospital and its infections obtained in hospital but the disease through the consumption of poultry have been reported. Recent studies have reported out breaks of Clostridium difficile in animal-based food sthatactas a source of in fection for humans. The purpose of this study was the incidence of Clostridium difficile in chicken meats old in Isfahan-Iran. In this study, a total of 120 raw meat samples of chicken meats old by the city of Isfahan-Iran were purchased randomly then transferred to the laboratory of Infectious and Tropical Diseases Research Center of Isfahan University, were evaluated of Clostridium difficile by the enrichment method and culture on CDMN medium in anaerobic conditions and biochemical tests and observed with hot staining. In this study, total of120 cases of Clostridium difficile contamination of chicken meat was studied. Among 120 meat samples examined, 19 samples (83/15%) were carriers of Clostridium difficile. Clostridium difficile in chicken meats old in Isfahan-Iran is isolated and thus there is a potential risk to humans through microorganisms especially meat consumption.