Effects of ~(18)F-FDG on the growth of Lewis lung cancer xenografts in C57BL/6 mice

Model of Lewis lung cancer xenograft in C57BL /6 mouse were set up with 18 mice,which were randomly divided into three groups: high-dose 18F-FDG group,low-dose 18F-FDG group and control group.The control was given 0.2 mL saline by intraperitoneal injection 18.5×107Bq and 9.25×107 Bq 18F-FDG in 0.2 mL saline was given intraperitoneally for one time on the 7th day after Lewis lung cancer cells were inoculated in the high dose 18F-FDG group and low-dose 18F-FDG group,respectively.The effects of 18F-FDG on animals and xenografts were investigated.After 22 days,expression of Bcl-2 and survivin proteins was detected using immunohistochemistry,and apoptotic rate were determined by flow cytometry.The results showed that the inhibition rate and apoptotic rate of xenografts in 18F-FDG-treated groups were higher than that in control group,and the expression of Bcl-2 and Survivin proteins in 18F-FDG-treated groups were less than that in control group(P0.05).Compared with low-dose 18F-FDG group,there were more significant differences between the high-dose 18F-FDG group and control(P0.01).It can be suggested that18F-FDG could effectively inhibit the growth of xenografts.The mechanism may be related to apoptosis caused by the down regulation of bcl-2 and survivin gene expressions in xenografts.