FRI0511 LOW ω6-ω3 RATIOS CAUSED REDUCTION IN CELLULAR INFLAMMATION IN HUMAN SYNOVIOCYTES CELL LINE (SW982): GENE EXPRESSION STUDY

Background Alarming global increase in osteoarthritis (OA), coupled with off target existing palliative care poses nutraceuticals an extremely attractive alternative in this respect. Beyond meeting the basic nutritional demands, nutrition is thought to play a beneficial role in the management of chronic diseases. Interestingly, the off target effects of nutriceuticals are beneficial in reducing co-morbidities. Skewed ratio of ω-6: ω-3 fatty acids (FAs) in modern diets has been shown associated with increased number of inflammatory diseases including bone and cartilage metabolism. Considering this, due to their disease modifying actions, ω-3 FAs are thought to serve better in OA management. Objectives We here investigated the effect of different ω-6: ω-3 ratios on synovitis, a prominent OA pathology. The selected ratios are generally found in global diet. Methods Selected ω-6FAs - arachidonic acid (AA), eicosadienoic acid (EDA), linolic acid (LA) and dihomo gamma linoleic acid (DGLA) along with selected ω-3 FAs docosapentaenoic acid 5 (DPA) and α-linolenic acid (ALA) were tested on induced SW-982 cells, in common dietary ratios of 16:1, 8:1, 4:1, 2:1 and 1:1 for their effect on synovial inflammation. For induction, 50ng/ml tumor necrosis factor-α (TNF-α) was used for 72 hrs and the PUFAs were added in the culture media in the selected ratios (concentration of each PUFA - 50μM). Finally, isolated cDNA was used to run quantitative RT-PCR for superoxide dismutase (SOD), interleukin-15 (IL-15), matrix metalloproteinase-1 (MMP-1) and vascular endothelial growth factor (VEGF). Results All the ratios LA:ALA of caused a remarkable reduction in MMP-1(P Conclusion Consequence of synovial inflammation in typical and atypical forms results in cartilage-loss, osteophytes and pain. FA ratios when used in equivalent, was found highly effective against IL 15, MMP1 and VEGF, whereas inflammation increases with increase in their proportions. Low SOD indicated lower oxidative-stress while it was up-regulated in response to high stress. FAs work in two distinct ways, membrane incorporation and metabolic modulation. Unsaturated fatty acid increase membrane fluidity and thus improves cell signaling. Analysis of treated and untreated cells showed incorporation of FAs in cell membrane, which improves membrane fluidity and status of signal transduction. Acknowledgement This research work has been carried out as a part of OAIBGE2016-18, an international consortium supported by INNO-INDIGO (EU-India multilateral research calls). Authors acknowledge EC of INNO-INDIGO for the funds. Disclosure of Interests None declared