Optimization of Ultrasound-assisted Enzymatic Polysaccharide Extraction from Turpiniae Folium Based on Response Surface Methodology

ABSTRACT Objective To optimize the extraction procedure and obtain the maximum total polysaccharide yield from Turpiniae Folium . Methods Response surface methodology (RSM), combining Plackett–Burman design (PBD), steepest ascent method, and Box–Behnken design (BBD), was employed. The significant factors contributing to polysaccharide production were determined by PBD. The path of steepest ascent method was performed to rapidly reach the neighborhood of the optimum region. BBD and RSM were applied to further investigate the mutual interaction between the variables and to define the optimal values. Results The significant factors contributing to polysaccharide production were enzyme concentration, extraction time, and liquid-to-solid ratio. The optimal conditions for maximal TFP were: enzyme concentration of 2.8 %, extraction time of 41 min, and liquid-to-solid ratio of 27 mL/g. The experimental yield of 3.08% was in good agreement with the model-predicted yield of 3.1% under the optimized conditions. Conclusion This study was successfully applied to optimize the extraction conditions of Turpiniae Folium polysaccharide, which can contribute to its further production and application.