Growth Characteristics of Sprouts and Changes of Antioxidant Activities in Common bean (Phaseolus vulgaris L.) with Cultivated Temperature

ABSTRACT The changes of growth characteristics and antioxidant activity for selection of optimum germinated temperature on common bean ( Phaseolus vulgaris L.). Common beans (IT100888, IT102849, and IT231267) were cultivated at 20℃, 23℃ and 25℃ during 5 days of germination. The range of whole length, hypocotyls length, thickness, abnormal germination and yield rate of sprouts was 7.27~27.62 cm, 3.10~18.86 cm, 1.80~2.27 mm, 5.54~18.34% and 205.95~ 618.71%, respectively. Antioxidant activities of common beans with germination temperature investigated. Common beans (IT100888, IT102849, and IT231267) germinated at 20℃, 23℃ and 25℃ during 5 days, and then extracted with 80% ethanol, and analyzed for total polyphenol content, DPPH and ABTS radical scavenging activity. Total polyphenol content increased from 474 mg GA eq/100 g sample for IT231267 to 1364 mg GA eq/g sample for 23℃ of germination. DPPH radical scavenging activity of IT102849 increased from 189mg Trolox eq./100 g sample (20℃) to 1073mg Trolox eq./100 g sample (23℃) also ABTS radical scavenging activity of IT234267 increased from 479 mg Trolox eq./100 g sample (20℃) to 1134 mg AA eq/100 g (23℃). These results suggest that germination temperature for increasing antioxidant activities may be 23℃.