Mobilization of P{EP}EP3313 artificial transposon in the germline of Drosophila melanogaster.

One of the most efficient and versatile mutagenesis methods used in Drosophila melanogaster (the fruit fly) relies on the mobilization of artificial derivates of P transposon. Aiming to reveal the biological significance of the patterns of transposition specific to P mobile element, mobilization of P{EP} artificial derivative was induced with a ?2-3 source in both male and female germline of D. melanogaster. By transposition of the viable and fertile insertion P{EP}3313, located in 68B1 at 592 pb downstream from 3’UTR of CG6199 gene, 46 new transgenic strains were constructed, each of them containing diverse reinsetions of P{EP}. Here we describe a combination of genetic and molecular experiments aiming to detect if conservation of the original insertion P{EP}EP3313 is associated with reinsertions in these mutant lines. A triplex PCR screening identified 22 mutant lines with conservation of P{EP}EP3313, a figure standing for 47,83 % of the transposition phenomena taken into account.