Community Structure and Kinetics Characterization of Enriched Nitrifiers Cultivated with Reject Water

The community of nitrifiers cultivated with reject water were investigated by fluorescent in situ hybridization (FISH) performed with 16S rRNA-targeted oligonucleotide probes,and the kinetic properties such as temperature correction factor τN and the half saturation coefficient KN were also investigated. The results showed that the percentage of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in the activated sludge was 15.7%±3.7% and 12.9%±3.2%,Nitrosomonas europaea-Nitrosococcus mobilis lineage were the dominant AOB,Nitrobacter spp. were the dominant NOB. The temperature correction factors of ammonium utilized rate (AUR) and nitrite utilized rate (NUR) were 1.092 and 1.061 respectively; the KN of AOB and NOB were (1.60±0.29) mg/L (NH+4-N ),(2.78±0.30) mg/L (NO-2-N ) respectively.