Vitagenic Effect of Specific Bioactive Fractions of Rhodiola with Trachurus sp. Extract Against Oxidative Stress Induced Aging in Human Amnion Derived Epithelial Cell Line: In View of a Novel Senolytic

Background Rhodiola rosea, an herb which has been used in traditional medicine for several years and LF, a class of lipoproteins, derived from the fish Trachurus sp. (LF-T) exhibits known anti-inflammatory activity. Objective Investigating the anti-aging effect of Rhodiola Specific Bioactive Fractions cluster in combination with LF-T (R-L compound) in H2O2 mediated oxidative stress induced human amnion derived epithelial cell line - FL cells. Methods FL cells treated with H2O2 to induce cellular aging, followed by treatment of R-L compound to study its antiaging characteristics. Based on the proliferation rate, 0.05% and 0.1% concentration of R-L compound was determined using MTT assay. Anti-aging and anti-oxidant assays - ABTS, DPPH, Hyaluronidase activity Nitric Oxide, Lipid Peroxidase and Superoxide Dismutase were performed. qPCR for anti-aging genes and matrix metalloproteinase genes were analyzed. Results FL cells treated with R-L compound exhibited with increased proliferation rate and free-radical reduction. Decreased Hyaluronidase enzyme activity and regulation of genes such as SIRT1, KLOTHO, SERPINA 6, MMP 9 and MMP 2 expression depicts the anti-aging role of R-L compound. Chemometric profiling of the R-L compound revealed that aromatic compounds and unsaturated fatty acids along with their derivatives were present predominantly which might have attributed for the potent oxidative stress impeded aging activity. Conclusion Specific Bioactive Fractions of Rhodiola in combination with LF-T obtained from Trachurus sp. involves in the regulation of aging genes and might be a novel approach to prevent the cells from oxidative stress damage and also it might avert the aging of cells.