Performance, digestion, nitrogen balance, and emission of manure ammonia, enteric methane, and carbon dioxide in lactating cows fed diets with varying alfalfa silage-to-corn silage ratios

Abstract Two trials were conducted simultaneously to study the effects of varying alfalfa silage (AS) to corn silage (CS) ratio in diets formulated to avoid excess protein or starch on lactating dairy cow performance, digestibility, ruminal parameters, N balance, manure production and composition, and gaseous emissions [carbon dioxide (CO 2 ), methane (CH 4 ), and ammonia-N (NH 3 -N)]. In trial 1 all measurements, except gas emissions, were conducted on 8 rumen-cannulated cows in replicated 4×4 Latin squares. In trial 2, performance and emissions were measured on 16 cows randomly assigned to 1 of 4 air-flow controlled chambers in a 4×4 Latin square. Dietary treatments were fed as total mixed rations with forage-to-concentrate ratio of 55:45 [dietary dry matter (DM) basis] and AS:CS ratios of 20:80, 40:60, 60:40, and 80:20 (forage DM basis). Measurements were conducted the last 3d of each 21-d period. Treatments did not affect DM intake, DM digestibility, and milk/DM intake. However, responses were quadratic for fat-and-protein-corrected milk, fat, and protein production, which reached predicted maxima for AS:CS ratio of 50:50, 49:51, and 34:66, respectively. Nitrogen use efficiency (milk N/N intake) decreased from 31 to 24g/100g as AS:CS ratio increased from 20:80 to 80:20. Treatments did not alter NH 3 -N/milk-N but tended to have a quadratic effect on daily NH 3 -N emission. Treatments had a quadratic effect on daily CH 4 emission, which was high compared with current literature; they influenced CH 4 emission per unit of neutral detergent fiber (NDF) intake and tended to influence CO 2 /NDF intake. Ruminal acetate-to-propionate ratio and total-tract NDF digestibility increased linearly with increasing AS:CS ratio. In addition, as AS:CS ratio increased from 20:80 to 80:20, NDF digested increased linearly from 2.16 to 3.24kg/d, but CH 4 /digested NDF decreased linearly from 270 to 190g/kg. These 2 counterbalancing effects likely contributed to the observed quadratic response in daily CH 4 emission, which may have been influenced also by increasing starch with increasing CS in the diet as reflected by the increased ruminal propionate molar proportion. Overall, production performances were greatest for the intermediate AS:CS ratios (40:60 and 60:40), but daily excretion of urine, manure, fecal N, urinary urea N, and urinary N decreased with increasing proportion of CS in the diet, whereas daily CH 4 emission was reduced for the 2 extreme AS:CS ratios (20:80 and 80:20). However, the proportion of AS and CS in the diet did not affect CH 4 /fat-and-protein corrected milk.