A novel amidase Azl13 hydrolase preparation and application superfamily

The present invention discloses a novel nitrilase enzyme amidase preparation and application Azl13 superfamily. Azl13 amidase amino acid sequence as shown in SEQ ID NO.1, an aryl group having an acyl amidase activity. Nucleotide sequence encoding a gene of amidase Azl13 NO.2 as shown in SEQ ID. By connecting a gene encoding the amidase Azl13 prokaryotic expression vector to construct a recombinant expression vector The recombinant expression plasmids were transformed into E.coli, and induced by IPTG to give amidase Azl13. Amidase Azl13 the present invention may be used in treatment anilines amides environmental pollutants, especially propanil. The present invention uses genetic engineering bacteria amidase Azl13, short production period, low cost, without environmental pollution, suitable for large scale cultivation; lactamase Azl13 the present invention have broad application prospects in terms of environmental pollution control pesticide.