Method for identifying cell sorting efficiency of different immunomagnetic bead cell separators

The invention discloses a method for identifying the cell sorting efficiency of different immunomagnetic bead cell separators. Lymphocyte CD3 antibodies marked with immunomagnetic beads serve as indexes, the percentage content of CD3+ positive cells is detected and analyzed with a flow cytometry, the relative percentage is worked out with a normalization method, the separation effects of the magnetic separators with different intensities for sorting the blood lymphocyte of multiple persons are compared, and thus the purpose of selecting the separator with the optimal magnetic field for sorting various cells is achieved. Due to the fact that the whole blood lymphocyte is wide in source, the method of detecting CD3+ cells with the flow cytometry is easy and convenient to implement; CD3+ is sorted with the immunomagnetic beads, operation is easy, and the cell separating capabilities of the immunomagnetic bead cell separators with different magnetic field intensities can be conveniently and quickly identified. A quick, convenient and effective selecting method can be provided for the immunomagnetic bead separator for sorting cells.