豬環狀病毒第二型ORF1、ORF2、ORF3基因重組蛋白的表現及純化

Porcine circovirus type2 (PCV2), a pathogen that infects the pig's respiration system, lymphoid tissues, kidney and liver, was the cause of porcine post-weaning multisystemic wasting syndrome. Recently, PCV2 caused the economic lose in America, Canada and Europe. Herein we extracted the nucleic acid of the suspect infected pigs from south of Taiwan in 1998 and the three genes (ORF1, viral replication related, ORF2, viral structure protein related and ORF3, function not clear) were all amplified by polymerase chain reaction. After the purification process, the PCR products were cloned into a E. coli expression vector, pET24a. After Sequences confirmation, the log phase cultures were induced by IPTG and the recombinant proteins were analyzed by SDS-PAGE. The results show that ORF1 and ORF3 genes could be highly expressed, besides ORF2. The recombinant proteins were confirmed by western blots. The purified recombination proteins should be the base for development of PCV2 antibody detection kit.