Phenotypic and genotypic characteristics of a Carbapenem-resistant Serratia marcescens cohort and outbreak: describing an opportunistic pathogen

Abstract Serratia marcescens is an emerging opportunistic pathogen with high genetic diversity. We described microbiological characteristics of isolates and also risk factors for infections caused by carbapenem-resistant S. marcescens(CrSm). A retrospective study of patients colonized(N=43) and infected(N=20) by CrSm over a three-year period was conducted. Polymerase chain reaction(PCR) for carbapenemases genes and molecular typing of all available strains was performed; Forty-two isolates were analyzed, including three environmental samples identified during an outbreak. Thirty-five CrSm carried blaKPC-2; one isolate was blaNDM-positive and four isolates carried blaOXA-101. The genomes were grouped into three clusters with 100% bootstrap; three patterns of mutations on ompC and ompF were found. The strains carried virulence genes related to invasion and hemolysis and the environmental strains presented less mutations on the virulence genes than clinical strains. The multivariate analysis showed that previous use of polymyxin(p=0.008) was an independent risk factor for infection by CrSm. Our study highlighted that blaKPC-2 in association with ompC, ompF mutation was the most frequent mechanism of resistance in our hospital, and that previous use of polymyxin was an independent risk factor for CrSm. There is a predominant clone, including the environmental isolates, suggesting that cross-transmission was involved in the dissemination of this pathogen.