Synthesis of enantiomeric selective α1-acid glycoprotein (AGP)-bonded capillary and its application in capillary electrophoresis

Abstract α 1 -Acid glycoprotein (AGP) was bonded to a fused silica capillary using 3-(2,3-oxopropoxypropyl, trimethylsilane as bridging reagent. The capillary can be used for the direct enantiomeric separation of some basic pharmaceuticals in capillary zone electrophoresis. The related operation parameters such as pH, salt concentration, organic modifiers in the buffer have been studied. It is demonstrated that an AGP-bonded capillary could be used in the different buffer solutions and under operation conditions in capillary electrophoresis with good stability.