In Vitro Anti Proliferative Activity, Antioxidant Potential and Total Phenolic Compounds of Black Tea Extract

Natural products, mainly isolated from medicinal plants, have considered as valuable sources for herbal anticancer drugs. The present study aimed to determine total phenolic and flavonoid contents, antioxidant activity, and anti proliferative activity of black tea (Camellia sinensis Kuntze) extract in vitro. Crude ethyl alcohol extract of black tea was prepared. To determine antioxidant activity, total phenol, and flavonoids content of the extract, the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu method and aluminum chloride colorimetric method were used, respectively. The anti proliferative activity in cancerous (AGS) and normal (HDFs) cell lines was tested by MTT 3-(4, 5-dimethylthiazol-2ol) 2, 5 di phenyl tetrazolium bromide] assay. The IC50 of DPPH radical assay was 8 +/- 1.41 mu g/ml, compared with butylated hydroxytoluene (BHT) with IC50 25.41 +/- 1.89 mu g/ml. The total phenol and flavonoid contents were 341.8 +/- 4.41 mg GAE/g and 21.1 +/- 2.11 mg RUT /g, respectively. The extract showed higher anti proliferative activity against the cancer cell line than normal cell line. 48 hours after treatment, crude ethyl alcohol extract inhibited the proliferation of AGS and HDFs cells with IC50 values of 264.3 mu g/ml and 689.5 mu g/ml, respectively. This study revealed that the crude ethyl alcohol extract of black tea suppresses the proliferation of gastric carcinoma cells. It is a rich resource of natural antioxidants and can prevent a lot of diseases such as cancer.