Optimization, reconstruction and heterologous expression of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri in Escherichia coli and its successive hydroxylation of toluene and benzene

Bo Wang,Feng Gao,Jing Xu,Gao Jianjie,Li Zhenjun,Wang Lijuan,Zhang FuJian,Yu Wang,Yongsheng Tian,Rihe Peng,Quan-Hong Yao

Optimization, reconstruction and heterologous expression of the gene cluster encoding toluene/o-xylene monooxygenase from Pseudomonas stutzeri in Escherichia coli and its successive hydroxylation of toluene and benzene

2021

Bo Wang
Feng Gao
Jing Xu
Gao Jianjie
Li Zhenjun
Wang Lijuan
Zhang FuJian
Yu Wang
Yongsheng Tian
Rihe Peng
Quan-Hong Yao

Biological treatment is a cost-effective and environmentally friendly technique for treating toluene. However, this process is limited by the type and quantity of toluene degraders that can be appl...

Keywords:

Heterologous expression
Pseudomonas stutzeri
Hydroxylation
Toluene
Chemistry
Gene cluster
Escherichia coli
Stereochemistry
Benzene
Toluene o-xylene monooxygenase

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