Simultaneous Determination of Peoniflorin, Campanulin and Ferulic Acid in Kangmin Granules by HPLC

OBJECTIVE: To establish an HPLC method for simultaneous determination of peoniflorin,calycosin and ferulic acid in Kangmin granules.METHODS: The separation was performed on a Kromasil C18 column (250 mm×4.6 mm,5 μm),and the mobile phase consisted of methanol-acetonitrile-0.4% phosphonic acid (20 ∶ 10 ∶ 70) with flow rate of 1.0 mL·min-1.The detection was performed at wavelength of 254 nm.Column temperature was room temperature and the injection volume was 10 μL.RESULTS: The linear ranges of peoniflorin,calycosin and ferulic acid were 180～3 600 μg·mL-1(r=0.999 2),4.6～92.0 μg·mL-1(r=0.999 1) and 8.0～160.0 μg·mL-1(r=0.999 4),respectively.The average recoveries were 98.3%(RSD=1.8%,n=9),99.0% (RSD=2.0%,n=9) and 100.5%(RSD=1.1%,n=9),respectively.CONCLUSION: The method is simple,accurate,sensitive and reproducible for the quality control of Kangmin granules.