Bungsteroid A, One Unusual C34 Pentacyclic Steroid Analogue from Zanthoxylum bungeanum Maxim.

Bungsteroid A (1), possessing an unreported carbon skeleton, was isolated from the pericarps of Zanthoxylum bungeanum Maxim.. It represents the first carbon skeleton of a C34 steroid analogue featuring a unique 6/6/6/6/5-fused pen-tacyclic skeleton, which has been determined by spectroscopic methods, quantum-chemical 13C NMR, ECD calculations, and calculations of optical rotations. Bungsteroid A showed the antiproliferative effects against HepG2, MCF-7 and HeLa cell lines with the IC50 values of 56.3 ± 1.1, 64.2 ± 0.9 and 74.2 ± 1.3 μM, respectively.