[Se-ZnCu-65P enhances mouse peritoneal macrophage phagocytosis and inhibits secretion of NO and H2O2 in vitro].

Objective To investigate the effect of selenopeptide on phagocytosis,NO and H_2O_2 secretion of mouse peritoneal macrophages. Methods Mouse peritoneal macrophages induced by lipopolysaccharide( LPS) were cultured for 24 hours by various concentrations of Se-Zn Cu-65 P,which is a selenopeptide with double antioxidant activity of superoxide dismutase( SOD) and glutathione peroxidase( GPx). Then,the relative cell activity was determined by methyl thiazolyl tetrazolium( MTT) assays,the phagocytic ability of macrophages was evaluated by neutral red uptake assay,nitric oxide( NO) content was examined by nitrate reductase method,and hydrogen peroxide( H_2O_2) content was detected by molybdate colorimetric method. Results When Se-Zn Cu-65 P acted on macrophages alone,the relative cell viability was enhanced,the phagocytic ability was not affected,H_2O_2 content was reduced,and NO level was almost unchanged. However,the phagocytic ability of macrophages induced by LPS was raised,meanwhile,secretion of NO and H_2O_2 was promoted. When Se-Zn Cu-65 P acted on macrophages induced by LPS,the phagocytic ability were further enhanced,and secretion of NO and H_2O_2 was inhibited significantly. Conclusion Se-Zn Cu-65 P can effectively improve the relative cell activity of mouse peritoneal macrophages induced by LPS,enhance the phagocytic ability of macrophages,and reduce the secretion levels of NO and H_2O_2.