Expressional level of human cervical cancer oncogene in primary hepatocellular carcinoma and its clinical significance

Objective To investigate the expressional level of human cervical cancer oncogene (HCCR) in primary hepatocellular carcinoma(PHC) and its clinical significance. Methods HCCR RNA in serum were detected by fluorescent quantitation reverse transcription polymerase chain reaction (FQ-RT-PCR) in 60 patients with HCC, hepatocirrhosis and normal health examination, and its correlation with the clinical pathological features was analyzed. Results The relative expression levels of HCCR RNA in the peripheral blood mononuclear(PBMC) of PHC patients were 0.515±0.092, and which in the hepatocirrhosis patients were 0.348±0.064, while HCCR RNA expression was not detected in the normal health examination, the difference was significant(P<0.05). FQ-RT-PCR results showed that the detection rate of PHC was the highest(83.33%), the second was hepatocirrhosis group(8.33%), while that of the normal health examination group was 0.00%, and the expression of HCCR RNA was strongly correlated with AFP expression quantity, portal thrombosis, tumor differentiation degree and TNM stage (P<0.05). Conclusions The HCCR mRNA expressional levels of PBMC in patients with PHC, hepatocirrhosis increase significantly, which show that HCCR may participate in the occurrence of PHC and is correlated with the evolution of malignant change in the PHC. Key words: Human cervical cancer oncogene; Primary hepatocellular carcinoma; Hepatocirrhosis