Development of a Novel BIA Enzyme Calorimetric Biosensor and Detection System for Hydrogen Peroxide Determination at Hazardous Level

This paper presents a calorimetric biosensor and detection system based on a medical thermistor and a disposable polymeric reaction cell for detecting hydrogen peroxide in a batch-injection-analysis (BIA) process. Single thermistor was used as a calorimetric sensing element to measure temperature change in an enzyme-substrate exothermic reaction. The detection system consists of a transducer, an analog-to-digital conversion device with 16-bits resolution, a single chip microcontroller, and a universal-serial-bus (USB) interface to communication with personal computer. A computer program for dynamic data acquisition and recording was developed to monitor and record temperature profile of the calorimetric biosensor during reaction. Disposable PMMA polymeric cell was used as a reaction cell to fill enzyme and the reaction medium. As results, after linearization of calorimetric biosensor and primary grade calibration, the accuracy of temperature measurement for the developed system is ± 0.001 °C and correlation coefficient R2 is 0.999 at temperature between 20 and 30 °C. When the calorimetric biosensor was incorporated with the detection system, it showed a linear range between 3.125 x 10-2 M to 1 M for hydrogen peroxide concentration detection with a correlation coefficient R2 of 0.9991. Compare with other types of hydrogen peroxide sensor and detection systems, the developed BIA enzyme calorimetric biosensor and detection system has a potential of low cost and high precision for hydrogen peroxide determination for the concentration at toxic and hazardous level. The system could be applied further for other biocalorimetry researches.