Reproducibility of single-pass donor DSAEK tissue preparation with the MORIA single-use microkeratome.

Summary Purpose To assess the reproducibility of single-pass cutting for preparation of ultra-thin (≤ 120 μm) donor cornea grafts in Descemet stripping automated endothelial keratoplasty (DSAEK). Methods All consecutive patients of DSAEK performed using the MORIA One Use microkeratome (MORIA, Antony, France) in between June 2014 and August 2015. Patient and donor central corneal thickness (CTT), microkeratome head, remaining stromal bed thickness (RSBT), number of cut and graft thickness at 1 month and 6 months postoperatively were recorded in this single-center prospective study. Results The mean preoperative donor graft CCT was 569.0 ± 45.1 μm and the mean donor endothelial graft CCT immediately after the cut was 116.5 ± 28.7 μm. At 1 month postoperatively, the mean CCT was 102.8 ± 35.9 μm. At 6 months, the mean CCT was 89.4 ± 26.2 μm. In all eyes, the mean CCT decreased from the post-cut (116.5 ± 28.7 μm) to the last visit (89.4 ± 26.2 μm) ( P Conclusion Single-pass technique with the MORIA One Use microkeratome offers safe and reproducible DSAEK tissue preparation and allows achieving ultra-thin DSAEK in 89.8% of cases.