Effects of taurine on apoptosis in neonatal rat cardiomyocytes induced by exogenous hydroxyl free radical

Objectives To obserev effects of taurine on apoptosis in neonatal rat cardiomyocytes induced by exogenous hydroxyl free radical. Methods ①The second passage of cultured neonatal rat cardiomyocytes were divided randomly into four groups and were co-cultured with hydroxyl free radical (0.1 mmol·L -1 ) and taurine (30 mmol·L -1 ) in serum-free medium for six hours and 24 hours respectively. The survival ratio and morphology of cardiomyocytes and the percentage of Annexin V +/PI - cells by flow cytometry were evaluated. Results ①The survival ratio of cardiomyocytes significantly decreased with 0.1 mmol·L -1 hydroxyl free radical ( P0.05 vs control) and significantly increased with taurine (30 mmol·L -1 ). ②The percentage of Annexin V +/PI - cells with flow cytometry increased significantly with 0.1 mmol·L -1 hydroxyl free radical and decreased with taurine ( P0.05 ).Conclusions Taurine attenuates apoptosis of neonatal rat cardiomyocytes induced by exogenous hydroxyl free radical.