Biodegradation of Tetrahydrofuran by Combined Immobilized of Pseudomonas oleovorans DT4

A new composite matrix,calcium alginate(CA) coupled with activated carbon fiber(ACF) was designed to immobilize the cells of Pseudomonas oleovorans DT4 for tetrahydrofuran(THF) degradation.The average removal rate of the CA-ACF immobilized cells reached 24.0 mg·(L·h)-1 with an initial THF concentration of 360 mg·L-1 when the concentration of CA and ACF was 3% and 1.5% respectively.The mechanical strength of the mobilized cells was also significantly improved with the addition of ACF.Compared to the free suspended cells,higher stable removal efficiency(more than 80%) of CA-ACF cells was detected under different conditions of temperature and pH.The feasibility of the newly designed matrix was also reflected by the repeated batch degradation which showed that the removal activity decreased insignificantly after 80 cycles with the modified reaction system(PNS).