Integrative Analysis in Oral Squamous Cell Carcinoma Reveals DNA Copy Number-Associated miRNAs Dysregulating Target Genes

2012 
ObjectiveTo better understand possible mechanisms involved in the dysregulation of gene expression unique to oral squamous cell carcinoma (OSCC) metastasis, the investigators examined the differential expression of microRNAs (miRNAs) in OSCC metastasis and their functional impact on target gene expression.Study DesignObservational assessment of DNA copy number, miRNA, and RNA expression in primary and metastatic OSCC.SettingUniversity of Washington Medical Center and affiliated hospitals.SubjectsTumor samples were taken from patients with primary incident OSCC; cells were laser-capture microdissected from 17 nonmetastatic primary tumors and 20 metastatic lymph nodes.MethodsDNA copy number aberrations and gene expression profiles were previously determined using Affymetrix 250K Nsp I SNP arrays and HU133 plus 2.0 expression arrays. miRNAs were interrogated with Exiqon’s Ready-to-Use PCR Panels assessing the expression of 368 human miRNAs.ResultsInvestigators found 31 miRNAs differentially expressed between...
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    35
    References
    17
    Citations
    NaN
    KQI
    []