Selective RNA Interference and Gene Silencing Using Reactive Oxygen Species-responsive Lipid Nanoparticles

Lipid-complexed small interfering RNA (siRNA) nanoparticles are promising gene regulation materials present excellent genetic but little cellular selectivity. Herein, we report a chemical strategy to enhance the gene silencing selectivity of these nanoparticles against cancer cells through covalent integration of reactive oxygen species (ROS)-degradable thioketal into lipid nanoparticle. These lipid nanoparticles can efficiently deliver siRNA into cells, and selectively silence cancer cell gene expression in response to the high level of intracellular ROS of cancer cells.