Neuroblast entry into quiescence is regulated intrinsically by the combined action of spatial Hox proteins and temporal identity factors
2008
Neural stem cell quiescence is an important feature in invertebrate and
mammalian central nervous system development, yet little is known about the
mechanisms regulating entry into quiescence, maintenance of cell fate during
quiescence, and exit from quiescence. Drosophila neural stem cells
(called neuroblasts) provide an excellent model system for investigating these
issues. Drosophila neuroblasts enter quiescence at the end of
embryogenesis and resume proliferation during larval stages; however, no
single neuroblast lineage has been traced from embryo into larval stages.
Here, we establish a model neuroblast lineage, NB3-3, which allows us to
reproducibly observe lineage development from neuroblast formation in the
embryo, through quiescence, to the resumption of proliferation in larval
stages. Using this new model lineage, we show a continuous sequence of
temporal changes in the neuroblast, defined by known and novel temporal
identity factors, running from embryonic through larval stages, and that
quiescence suspends but does not alter the order of neuroblast temporal gene
expression. We further show that neuroblast entry into quiescence is regulated
intrinsically by two independent controls: spatial control by the Hox proteins
Antp and Abd-A, and temporal control by previously identified temporal
transcription factors and the transcription co-factor Nab.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
59
References
98
Citations
NaN
KQI