Transcriptome analysis of wolf spider Pardosa laura exposed to beta-cypermethrin

To understand the molecular mechanism of Pardosa laura in response to beta-cypermethrin (beta-CP) stress, next generation RNA-seq technique was employed to analyse the expression patterns of resistance- and stress-response genes in P. laura subjected to beta-CP. De novo sequence assembly yielded 49,003 unigenes, 20,912 of which were annotated in the public databases. A total of 370 up or down-regulated genes altered by beta-CP were detected, among them, 318 unigenes were upregulated, including one GST, one GPx, five CYP450s, two HSPs (one HSP70 and one HSP20) and two ABC transporters. Functional enrichment analysis revealed that carbon metabolism, glutathione metabolism, drug metabolism-CYP450, oxidative phosphorylation, as well as lysosome, phagosome and mTOR signaling pathway were predominately enriched in response to beta-CP stress. Certain critical genes involved in detoxification were detected to be differentially expressed between the beta-CP treated and control spiders. These results provide a fundamental resource for further research in the molecular characterisation of P. laura responding to pesticide.