Epidemiological investigation of community-acquired methicillin resistant Staphylococcus aureus infection from 4 hospitals in Beijing

2012 
Objective To investigate the prevalence, antibiotic characteristics as well as molecular background of community-associated methicillin-sensitive Staphylococcus aureus (CA-MRSA) from patients with skin and soft tissue infections from 4 different hospitals in Beijing. Methods Five hundred and one patients were enrolled from 4 hospitals prospectively.Patients with skin and soft tissue infections and no risk factors for healthcare-associated acquisition were included.Sample from the infection sites were collected for culture.Case report form was filled out for each patient.Antibiotic susceptibility test and molecular analysis was performed for each Staphylococcus aureus isolate. Results Totally 164 Staphylococcus aureus isolates were cultured from the patients with skin and soft tissue infections.Of them 5 isolates were CA-MRSA.These 5 CA-MRSA isolates harbored SCCmecⅠ, SCCmecⅢ, SCCmecⅣ,SCCmecⅤand untypable, respectively.CA-MRSA was highly resistant to β-lactamase, levofloxacin, erythromycin and clindamycin, but susceptible to vancomycin,teicoplanin, linezolid, daptomycin, and trimethoprim/sulfamethoxazole.Prevalence of PVL in community-associated methicillin sensitive Staphylococcus aureus(CA-MSSA) and CA-MRSA were 41.9% and 2/5.Other toxins expressed similarly between them.Combined with multilocus sequence typing(MLST) and spa typing, the major clones of CA-MSSA were ST398-t034, ST7-t796, ST398-t571, ST1-t127, and ST188-t189, while in CA-MRSA were ST239-t037-SCCmecⅠ, ST239-t632-SCCmecⅢ, ST59-t437-SCCmecⅤ, ST8-t008-SCCmecⅣ, and ST6-t701-NT. Conclusions The low prevalence of CA-MRSA in Beijing and complexity of the genetic background in CA-MRSA were observed.Clone spread is not found among CA-MRSA isolates.CA-MRSA exhibit higher resistance compared with methicillin sensitive Staphylococcus aureus (MSSA).Rational drug use scheme is called in the clinical practice to prevent development of high level resistance. (Chin J Lab Med,2012,35:237-242) Key words: Staphylococcus aureus; Staphylococcal infections; Soft tissue infections; Sequence analysis; Bacterial toxins
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    0
    References
    0
    Citations
    NaN
    KQI
    []