The action of proglumide blocking gastrin on gastric cancer cells

Objective To investigate the practicability of proglumide to treat gastric cancer. Methods MKN45 gastric cancer cell line was cultuered and the effects on gastrin and gastrin receptor antagonist proglumide proliferative rate,cell dynamic cycle distribution and the concentration of cAMP in the cells were obsered in vitro. Results Gastrin promoted the proliferation of MKN45 cells and shifted cells from phase G0/G1 to phase S,G2/M as well as increased intracellular cAMP,while proglumide blocked these effects. Conclusions Gastrin induces the proliferation and synthesis of DNA by its receptor. Proglumide may provide a new approach of noncytotoxic treatment of gastric cancer.