The Genetic Effect of Ghrelin Gene on Egg Performance in Gaoyou Duck

The genetic polymorphism of Ghrelin gene was detected by PCR-SSCP and DNA sequencing in Gaoyou duck.The genetic effects between Ghrelin gene and egg performance were analyzed.The results showed that: ①There were 9 bp deletions at 157 bp of intron 1,and the frequency of A/B allele of intron 1 was 0.24 and 0.76,respectively.The B allele gene had positive effect on egg production and body weight at first laying.BB and AB genotypes had higher egg production compared to AA genotype(P0.05).②There was T→C at 431 bp site,and the frequency of C/D allele of intron 2 was 0.41 and 0.59,respectively.The C allele gene had positive effect on body weight at first laying egg.CD genotype had higher egg production compared to DD genotype(P0.05).③There was A→G at 909 bp site which caused Thr→Ala change,and E/F allele of exon 3 was 0.33 and 0.67,respectively.The F allele gene had a positive effect on egg production and body weight at first laying,egg weight and highest clutch days.EF genotype had higher clutch days than EE and FF genotypes(P0.05).