HPLC (high performance liquid chromatography) measuring method for content of vitamin B6 in common wheat

The invention discloses a HPLC (high performance liquid chromatography) measuring method for content of vitamin B6 in common wheat. An extracting method comprises the following steps of preparing common wheat flour into whole wheat flour; under the condition of existence of glyoxylic acid and ferrous sulfate, performing enzymatic hydrolysis on the whole wheat flour in a sodium acetate water solution; after the enzymatic hydrolysis of the system is finished, derivatizing filtrate after filtering; adding acetic acid, oscillating and centrifuging, so as to obtain a supernatant, namely the vitamin B6, wherein the enzymatic hydrolysis adopts compound enzyme, and the compound enzyme is a compound of papain, acid phosphatase, alpha-amylase and reduced glutathione hormone. The method has the advantages that by combining the HPLC and a fluorescent detector, the sensitivity is higher, and the selectivity is higher; the vitamin B6 can exist in the pyridoxamine and pyridoxal types; by adding the glyoxylic acid and the ferrous sulfate, the pyridoxamine is converted into the pyridoxal, the pyridoxal is reduced into pyridoxine, and the stable fluorescence is produced under the excitation action of ultraviolet light, and is can be used for the detection of the fluorescent detector.