The Use of Acetone Precipitation in the Isolation of Ribosomal Proteins

Acetone precipitation is a rapid and efficient method for concentrating ribosomal proteins. This procedure also permits the elimination of sodium dodecylsulphate or urea from protein solutions containing these compounds. Acetone-precipitated 30-S ribosomal proteins can be used for the reconstitution of active 30-S ribosomal subunits.