Label-free monitoring of molecular binding based on extraordinary optical transmission with enhanced accuracy
2016
This paper describes a preliminary study of the anti-HIV antibody 10E8 and Membrane Proximal External Re Gion (MPER) antigen's interaction based on extraordinary optical transmission (EOT) enabled by a free-standing gold film perforated with periodically arrayed nano-holes. Particularly, the spectral shift caused by the microfluidic flow rate through the porous and suspended Au/Cr/Si3N4 film has been quantified for the first time and this shift as measurement error has been decoupled and eliminated from the recorded signal to enhance the monitoring accuracy.
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