Aflatoxins and Saccharomyces cerevisiae: yeast modulates the intestinal effect of aflatoxins, while aflatoxin B1 influences yeast ultrastructure
2017
The gastrointestinal tract (GIT) is the main site where absorption of food components takes place and the first system coming into contact with mycotoxins of dietary origin. The aim of this work was to study the effect of probiotic Saccharomyces cerevisiae RC016 on intestinal villi of rats exposed to aflatoxins for 60 days. Moreover, the effect of in vitro aflatoxin B1 (AFB1) exposure on yeast cell ultrastructure was evaluated. Six treatments were applied (n=6) to inbred male Wistar rats: (1) uncontaminated feed control (F); (2) yeast control; (3) F + 40 μg/kg AFB1 + 20 μg/kg aflatoxin G1 (AFG1); (4) F + 100 μg/kg AFB1 + 50 μg/kg AFG1; (5) F + 40 μg/kg AFB1 + 20 μg/kg AFG1 + daily oral dose 108 viable S. cerevisiae cells; and (6) F + 100 μg/kg AFB1 + 50 μg/kg AFG1 + daily oral dose 108 viable S. cerevisiae cells. Morphometric measurements (villus length and width, crypt depth, quantification of goblet cells) were assessed using image analysis. S. cerevisiae RC016 cells were exposed to 20 μg/ml of AFB1 in ...
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