СОСТАВ ЖИРНЫХ КИСЛОТ В ПОДКОЖНОЙ КЛЕТЧАТКЕ ЗДОРОВОГО ЧЕЛОВЕКА КАК РЕФЕРЕНСНЫЙ ПОКАЗАТЕЛЬ ДЛЯ ВЫЯВЛЕНИЯ ПРЕДИКТОРОВ ЗАБОЛЕВАНИЙ ЖЕЛУДОЧНО-КИШЕЧНОГО ТРАКТА

The Purpose. To analyze the composition of human subcutaneous adipose tissue in healthy individuals for the amount and composition of fatty acids. Material and methods. 10 samples of subcutaneous adipose tissue of different individuals from different anatomical regions have been studied. Adipose tissue was collected during liposuction from the abdomen, lumbar region and hips as the different samples, then it was centrifuged. The subject of the analysis was the upper part of lipoaspirate - pure fat that was released from the destroyed adipocytes. Pure fat samples were centrifuged and methylated by NaOH in methanol solution. Methyl esters were extracted by hexane and MTBE then analyzed on GC-MS Thermo Scientific Trace GC Ultra DSQ. The material was examined for 27 fatty acids. Results. In samples dominated fat oleic acid methyl ester C18:1n9c (42.3±2.2%) and palmitic acid C16:0 (22.5±3.4%). In less percent linoleic C18:2 (17.5±6.5%), stearic C18:0 (4.8±1.0%), palmitoleic C16:1t (3.0±0.6%), myristic C14:0 (2.2±0.5%) and eicosenoic C20:1n9 (0.8±0.2%) acids observed. It was found that the ratio of fatty acids in each sample was the same, they were different only in total amount. Conclusions. In human subcutaneous fatty tissue, regardless of the anatomic zones are more common oleic, methyl ester, palmitic, stearic and linoleic acids.