Влияние лираглутида на микроциркуляцию у крыс с экспериментальным сахарным диабетом, сопровождающимся абсолютной недостаточностью инсулина

Introduction . Liraglutide therapy has been shown successful in improving glycemic control in patients with type 2 diabetes, but the prospects of this drug use in treating patients with type 1 diabetes mellitus remain unclear. Therefore, the studies of liraglutide angiotropic effects have considerable scientific and practical interest. The aim of present study was to investigate the effect of glucagon-like peptide-1 receptors agonist – liraglutide – on microcirculation in conditions of alloxan-induced insulin deficiency in white rats. Materials and methods . The study was carried out in 40 white nonlinear rats that were divided into control (n=20), comparative (10 rats with alloxan-induced diabetes at a dose of 100 mg/kg) and experimental (10 animals with alloxan-induced diabetes that were treated with liraglutide) groups. Liraglutide at a dose of 0.4 mg/kg/day was injected subcutaneously in animals of the experimental group from Day 21 till Day 42 of the experiment. On Day 42 of the experiment, the concentrations of glucose and glycated hemoglobin in the blood in animals of the comparison and the experimental group were evaluated, as well as the microcirculation of dorsal skin of posterior paw was monitored with Laser Doppler Flowmetry (LDF). Results . Disorders of carbohydrate metabolism induced by alloxan in rats entailed pronounced changes in the microcirculation of posterior paw dorsal skin, associated with a decrease in endothelium-dependent vasodilation and increased neurogenic tone. Treatment with liraglutide caused normalization of glycemic metabolism and significantly (р<0,00001) increase perfusion of posterior paw dorsal skin in rats with alloxan-induced diabetes to 12,9 (12,4;13,4) perfusion units regarding to 9,6 (9,1; 10,1) in animals of the comparison group. Restoration of posterior paw skin microvascular perfusion under the influence of liraglutide was realized by stimulation of endothelium-dependent vasodilation. Conclusions . The administration of liraglutide in a dose of 0.4 mg/kg/day for 21 days in rats with alloxan diabetes leads to normalization of carbohydrate metabolism and restoration of normal perfusion of posterior paw dorsal skin.