A nutritional intervention study with hydrolyzed collagen in pre-pubertal spanish children: influence on bone modeling biomarkers.

Aim: The aim of the study was to investigate the infl uence of dietary intake of commercial hydrolyzed collagen (Gelatine Royal ® ) on bone remodeling in pre-pubertal children. Methods: A randomized double-blind study was carried out in 60 children (9.42 ± 1.31 years) divided into three groups according to the amount of partially hydrolyzed collagen taken daily for 4 months: placebo (G-I, n = 18), collagen (G-II, n = 20) and collagen + calcium (G-III, n = 22) groups. Analyses of the following biochemical markers were carried out: total and bone alkaline phosphatase (tALP and bALP), osteocalcin, tartrate-resistant acid phosphatase (TRAP), type I collagen carboxy-terminal telopeptide, lipids, calcium, 25-hydroxyvitamin D, insulin-like growth factor-1 (IGF-1), thyroid-stimulating hormone, free thyroxin and intact parathormone. Results: There was a signifi cantly greater increase in serum IGF-1 in G-III than in G-II (p < 0.01) or G-I (p < 0.05) during the study period, and a signifi cantly greater increase in plasma tALP in G-III than in G-I (p < 0.05). Serum bALP behavior signifi cantly (p < 0.05) differed between G-II (increase) and G-I (decrease). Plasma TRAP behavior signifi cantly differed between G-II and G-I (p < 0.01) and between G-III and G-II (p < 0.05).