Absence ofp15INK4Bandp16INK4AGene Alterations in Primary Cervical Carcinoma Tissues and Cell Lines with Human Papillomavirus Infection

Abstract Objective. Alterations of the p15 INK4B and p16 INK4A gene which are separated by 25 kb on chromosome 9p21 have been reported in various tumor-derived cell lines and primary tumors, but the role of these genes in cervical cancer is unknown. To determine the frequency of deletions and point mutations of these genes in human cervical cancer, we examined for alterations of the p15 INK4B and p16 INK4A genes in cervical carcinomas. Methods. We examined 57 primary tumors and matched normal tissues and 3 cervical cancer-derived cell lines. All the tumor tissues and cell lines were human papillomavirus (HPV) positive. Deletions or point mutations of exon 2 of the p INK4B gene and exons 1, 2, and 3 of the p16 INK3A gene were examined by polymerase chain reaction and direct sequencing, respectively. Results. Our data indicate no evidence for intragenic homozygous deletion or point mutation in the primary cervical cancer tissues or cancer-derived cell lines. Conclusion. Deletions or point mutations in the p15 INK4B or p16 INK4A gene may not be required for the development of HPV-positive cervical cancer or for establishment of cervical cancer cell lines.