Effects of Exogenous Salicylic Acid on the Antioxidative System in Bean Seedling Treated with Manganese.

In the present study we investigated the role of salicylic acid (SA) in regulating Mn-induced oxidative stress in bean (Phaseolus vulgaris) leaves. Exposure of plants to 100 μM Mn inhibited biomass production and intensively increased Mn accumulation in leaves. Concomitantly, Mn significantly enhanced protein carbonyl, H2O2 content and lipid peroxidation as indicated by malondialdehyde (MDA) accumulation. SA (10, 50 and 100 μM) pretreatment alleviated the negative effect of Mn on plan growth and led to decrease in oxidative stress induced by Mn stress. Furthermore, SA enhanced the activities of catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.11), but lowered that of superoxide dismutase (SOD, EC 1.15.1.1) and guaiacol peroxidase (POD, EC Original Research Article Saidi et al.; BBJ, 6(3): 93-100, 2015; Article no.BBJ.2015.031 94 1.11.1.7). The data suggest that the beneficial effect of SA could be related to avoidance of oxidative damage upon exposure to Mn thus reducing the negative consequences of oxidative stress caused by Mn toxicity.