EVALUATION OF DNA DAMAGING POTENTIALS OF LUMINOL, HEMASCEIN AND BLUESTAR USING THE COMET ASSAY

The regants such as Luminol, Hemascein and BlueStar are used to determine the presence of blood. The heme-group of hemoglobine acts as a catalyst and reacts with hhdrogen peroxide to produce blue light. The single-cell gel electophoresis (SCGE), also known as the Comet Assay, is a sensitive and simple technique used to measure deoxyribonucleic acid (DNA) strands breaks in eukaryotic cells. Electrophoresis of lysed cells results in comet-like images, observed and scored under fluorescene microscope.