Method for detecting HRP through luminol-hydrogen peroxide-bromophenol red-HRP-BSA chemiluminescent system

The invention relates to a method for detecting HRP through luminol-hydrogen peroxide-bromophenol red-HRP-BSA chemiluminescent system and aims to build a novel chemiluminescent system which is used for high sensitivity detection of HRP. The chemiluminescent system comprises liquid A, liquid B and liquid C, wherein the liquid A is mixed liquid of bromophenol red and hydrogen peroxide; the liquid B is luminol; the liquid C is a BSA aqueous solution; a sample HRP to be detected is diluted through the liquid C; the diluted sample is placed in a measuring cup and the liquid A is added for light shielding reaction; the liquid B is added and chemiluminescence signal strength determination is carried out in a weak chemiluminescent analyzer. The method has the advantages of higher sensitivity and better linearity on the aspect of HRP detection in comparison with a method adopting a classic luminol-hydrogen peroxide-HRP-PIP chemiluminescent system.