Determination of α-Chymotrypsin-Catalyzed Reaction Rates at Different Temperatures and PH Values by Raman Spectroscopy

The rates of an enzymatic reaction are determined using spontaneous Raman spectroscopy. Time dependences of integrated Raman intensities in spectra of the reaction mixtures are analyzed. The rates of the α-chymotrypsincatalyzed reaction are determined at different temperatures and pH values.