Single-tube Quantitative Rapid Detection for Coliform Bacteria Based on Enzyme Specific Technology

To detect coliform bacteria (CB) with simplicity and portability, a novel, single-tube quantitative rapid detection method was developed based on CB-specific enzymatic technology. This technology involved a simple multi-channel spectrometer which could detect 7 cfu mL-1 CB specifically, and the approach was applied to various food and water samples.