The Preventive Effect of Danxi Tongfeng Variation Formula on the Gouty Arthritis Induced by Microcrystalline Monosodium Urate(MSU) of Rabbits

Objective:To observe the preventive effect of Danxi Tongfeng variation formula on the gouty arthritis induced by microcrystalline monosodium urate(MSU) of rabbits and its mechanism.Methods:Gouty arthritis models of rabbits were established by using MSU.The number of leukocyte in the joint fluid was counted.And the quantities of cytokines and inflammatory factors were measured.HE stainings of joint and surrounding soft tissue were performed.Results:The high and middle dose of Danxi Tongfeng variation formula can significantly reduce the leukocyte count and the levels of TNF-α,PGE2,IL-1β,IL-8 in rabbit joint fluid.Also the edema of the joints and soft tissue,inflammatory infiltration as well as degeneration necrosis were also lessened.Conclusion:Danxi Tongfeng variation formula can obviously improve the MSU-induced gouty arthritis in rabbits.Its mechanisms are probably related to inhibiting the chemotaxis and activation of inflammatory cells,the synthesis and release of the inflammatory cytokines.