HIGH PERFORMANCE LIQUID CHROMATOGRAPHY OF BOVINE AND HUMAN CARBONIC ANHYDRASE, COMPONENTS A AND B

Bovine carbonic anhydrase [carbonate hydrolyase, EC 4.2.11] has been analyzed by high performance liquid chromatography, HPLC, using a TSK column, IEX-540 DEAE, eluted with 0.01 mol dm−3 phosphate buffer of pH 6.5. By means of this method, components A and B of bovine carbonic anhydrase, BCA(A) and BCA(B), showed peaks at tR=13.5 and 5.5 min, and the apo-BCA(A) and apo-BCA(B) showed peaks at tR=15.8 and 6.0 min, respectively. Under the same conditions, human CA(B) showed a peak at tR=2.4 min.