Quality assessment of diagnostic core-needle breast biopsy using structured illumination microscopy

Structured illumination microscopy provides a compelling solution to location inaccuracy a in core-needle breast biopsy procedures. Fluorescent H and E analog dyes and dual-channel SIM imaging were utilized to create an digital pathology image analogous to a standard histology within minutes for point of care pathology. This study validated SIM’s ability to generate an image for on-site pathology validation of sample quality which minimizes the risk of a repeat procedure. Diagnoses from the diagnostic-quality SIM images were also proven to align with diagnoses obtained by standard histological processing of the same sample.