Protective effect of Minocycline on Bax and Bcl-2 gene expression, histological damages and oxidative stress induced by ovarian torsion in adult rats, as an animal experimental study.

Background: The aim of this study is the investigation of apoptotic related gens in ovary of the rat model of torsion/detorsion treated by Minocycline. Materials and Methods: This experimental study performed on 32 female Wistar rat which classified into 4 group;1 sham; 2 TD: torsion/detorsion group received normal saline; 3 TDM: torsion/detorsion group treated with 40mg/kg Minocycline; 4 MC: healthy group received 40mg/kg Minocycline. After the therapy period (7days), histoplogical parameters, the oxidative stress markers in serum and the expression of apoptotic related genes were measured in the ovary of rats. Results: The level of SOD, GPX was decreased in the TD group. And also the level of MDA increased in the TD group and decreased in treated groups (p<0.001). The expression of Bax was elevated in the TD group although in treated groups their expression ratio was attenuated. Conclusion: Our result shows that the administration of Minocycline can replace the oxidative damage in ovarian tissue and regulate the apoptotic related genes expression.