ISSR Analysis for genetic diversity of Chrysanthemum morifolium

Objective To investigate the genetic diversity of Chrysanthemum morifolium and provide the evidence for evaluation and exploitation of C.morifolium germplasm.Methods The genetic diversities of 31 germplasm from different habitats were investigated with the technique of inter-simple sequence repeat(ISSR),which were 29 of C.morifolium,1 of C.indicum,and 1 of C.nankingense.Results Twenty-two primers were selected to produce highly reproducible ISSR bands.Among 182 amplified bands,149 showed polymorphism,the percentage of polymorphic bands(PPB) reached to 81.87%. Observed effective number of alleles(Ne),Nei's gene diversity index(He),and Shannon information index (Ⅰ) were 1.348 1,0.219 1,and 0.345 1,respectively.Conclusion ISSR Method is suitable for identification and genetic diversity analysis of C.morifolium.