Antioxidant Enzyme Activity and Antimicrobial Activity of Isatis tinctoria Extract

To gather the basic data for increasing the utilization of Isatis tinctoria, we examined the effects of both antioxidative enzyme activity and antimicrobial activity from the extract of Isatis tinctoria. Ascorbate Peroxidase activities reveal that there is an decrease in order; ethanol extract from its stem (1601.7 Unit/ mg protein), methanol extract from its leaf (1133.7 Unit/ mg protein) and distilled water extract from its leaf (524.3 Unit/ mg protein). Catalase activities reveal that there is an decrease in order; ethanol extract from its flower petal (177.1 Unit/ mg protein), methanol extract from its leaf (120.8 Unit/ mg protein) and distilled water extract from its flower petal (55.4 Unit/ mg protein). Peroxidase activities reveal that there is an decrease in order; ethanol extract from its flower petal (27.1 Unit/ mg protein), methanol extract from its flower petal (14.6 Unit/ mg protein) and distilled water extract from its stem (10.4 Unit/ mg protein). Superoxide dismutase activities reveal that there is an increase in order; distilled water extract from its root (90.8%), methanol extract from its flower petal (80.1%) and ethanol extract from its root (75.5%). Its flower extract showed a antimicrobial activity only against Vibrio parahaemolyticus, its root extract had only against Staphylococcus aureus, and its stem extract had against Bacillus subtilis, Escherichia coli and Staphylococcus aureus, regardless of solvents. Especially, distilled water extract from its leaf showed a high antimicrobial activity against both Bacillus subtilis and Escherichia coli and inhibition diameters against those were 30.0 and 24.0 mm, respectively.